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Shi S, et al. Acr is an acrosomal enzyme with chymotryptic activity that is released during the acrosome reaction. Despite these arguments, the view that genes essential for mammalian fertilization do exist has been confirmed by the discovery of proteins whose gene disruptions prevent fertility both in vitro and in vivo e. When the fertilized egg implants — congratulations — you have achieved pregnancy. The mechanism of the acrosome reaction itself has been well characterized. Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme Ace - and calmegin Clgn -deficient mice.